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Objective To investigate effects of total flavonoids of litchi (TFL) on the proliferation of rat hepatic stellate cells (HSC-T6) in comparison with western medicine rosiglitazone, and to explore the mechanism of anti hepatic fibrosis of TFL. Methods Effect of TFL on proliferation of HSC-T6 was examined by MTT. The expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) mRNA, connective tissue growth factor (CTGF) mRNA in HSC-T6 cells exposured were examined by real-time quantitative PCR. Effects on HSC-T6 CTGF protein from TFL and rosiglitazone were detected by Western bloting. Results The expression of PPAR-γ mRNA was upregulated and the expression of CTGF mRNA and protein was downregulated after exposure to TFL and rosiglitazone for 72 hours. And the effect of TFL increased with the increase of concentration. Conclusion TFL can inhibit the proliferation of HSC-T6 and antagonizing liver fibrosis. This mechanism may be associated with the upregulation of PPAR-γ expression and the downregulation of CTGF expression.
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ObjectiveTo explore the therapeutic effect and mechanism of dexamethasone combined with puerarin for treatment of paraquat (PQ) poisoned rats.Methods Thirty Sprague Dawley (SD) rats were divided by random number table into 5 groups: control, model, dexamethasone, puerarin and combined groups, 6 rats in each group. The PQ poisoned rat model was established by intraperitoneal injection of PQ 25 mg/kg (1 mL), while in the rat of the control group, the same volume of normal saline was injected intraperitoneally. After 2 hours, in the rat of dexamethasone group, 20 mg/kg dexamethasone in 1.5 mL was injected intraperitoneally, and in the rat of puerarin group, 100 mg/kg puerarin in 1.5 mL was injected intraperitoneally. In the rat of combined group, 20 mg/kg dexamethasone combined with 100 mg/kg puerarin in a total amount of 1.5 mL was injected intraperitoneally. In the control group and model group, 1.5 mL of normal saline was injected intraperitoneally. The above mentioned treatments were repeated once more 26 hours later. In 26-50 hours after modeling, urine was collected, and urine N-acetyl-beta-D-amino glycosidase enzymes (NAG) was tested; at the 50th, abdominal aortic blood was collected to test oxygen partial pressure (PaO2). The lung index, kidney index, and the levels of malonaldehyde (MDA), superoxide dismutase (SOD) and myeloperoxidase (MPO) were measured in the left lung and kidney tissue homogenates separately; the right lung and kidney were harvested to observe pathological changes under light microscope. Another 30 SD rats were treated the same as above measures but no sacrifice to observe 30-day survival rate in each group.Results The lung index, kidney index, NAG in urine, MDA and MPO levels in lung and kidney tissue homogenates in model group were significantly higher than those in the control group [lung index: 9.80±1.83 vs. 4.97±1.14, kidney index: 9.40±1.32 vs. 7.01±0.32, NAG (U·kg-1·day-1): 1.93±0.18 vs. 0.41±0.03, MDA of lung (nmol/mg): 1.04±0.15 vs. 0.28±0.10, MDA of kidney (nmol/mg): 1.39±0.16 vs. 0.66±0.13, MPO of lung (U/g): 1.14±0.08 vs. 0.81±0.06, MPO of kidney (U/g): 0.88±0.08 vs. 0.52±0.12]; while PaO2 [mmHg (1 mmHg = 0.133 kPa): 59.83±4.40 vs. 97.00±2.83] and SOD (U/mg): lung was 27.38±3.48 vs. 86.88±5.88; kidney was 24.18±3.74 vs. 57.86±6.14) were obviously lower than those in control group (allP 0.05).Conclusions Dexamethasone can improve the prognosis of rats with acute paraquat intoxication, it can provide lung protection markedly, but cannot provide significant protective effect on kidney; puerarin has therapeutic effect on rats with acute PQ poisoning, it can provide not only lung protection but also kidney protection. The effect of treatment with dexamethasone combined with puerarin is not superior to that by using dexamethasone or puerarin alone.
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OBJECTIVE:To compare the clinical efficacy and safety of amiodarone and propafenone in the treatment of chron-ic atrial fibrillation cardioversion. METHODS:60 patients with chronic atrial fibrillation cardioversion were randomly divided into propafenone group and amiodarone group. All patients were given conventional treatment,including treating primary disease,con-trolling symptoms,orally giving aspirin,intravenous infusion of GIK,monitoring heart rate,QT interval,P-R interval,QRS time,serum potassium and serum magnesium,etc. On this basis,propafenone group was orally given propafenone 450 mg for con-tinuous 3 months,3 times a day,and then the dose was decreased to 300 mg to maintain the sinus rhythm;amiodarone group was orally given amiodarone 200 mg for continuous 7 d a month,twice a day,and then the dose was decreased to 200 mg or 100 mg to maintain the sinus rhythm. The clinic data in 2 groups was observed,including clinical efficacy,simple drug cardioversion,elec-trical cardioversion,electrical cardioversion power,hospitalization time and incidence of adverse reactions,and the recurrence rate in 48 months was followed up. RESULTS:There were no significant differences in the clinical efficacy,simple drug cardiover-sion,electrical cardioversion,electrical cardioversion power,hospitalization time,incidence of adverse reactions and recurrence rate between 2 groups(P>0.05). CONCLUSIONS:Based on the conventinal treatment,amiodarone and propafenone have similar clinical efficacy and safety in the treatment of chronic atrial fibrillation cardioversion,and both of them can be used as the conven-tional drugs for treating chronic atrial fibrillation cardioversion.
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Objective To explore clinical efficacy of Shenlingbaizhu decoction on immune status in patients with ulcerative colitis(UC).Methods 60 patients with UC were selected and divided into control group and experiment group evenly and randomly.Control group received treatment of salazosulfapyridine,while experiment group received treatment of Shenlingbaizhu decoction based on treatments of control group.Clinical efficacy,serum levels of IL-6 and IL-8,percentage of Treg cells in peripheral blood were observed and compared between two groups before and after treatments.Results Total clinical efficacy of experiment group was significantly higher than that of control group(P＜0.05)(90.00％ vs 76.67％);Serum level of IL-6 was decreased significantly in all patients(P＜0.05),more significantly in experiment group(P＜0.05)after treatment;Serum level of IL-10 increased significantly in experiment group after treatment(P＜0.05);Percentage of Treg cells increased significantly in experiment group after treatment(P＜0.05).Conclusion Combination therapy of salazosulfapyridine and Shenlingbaizhu decoction shows significantly better clinical effects than salazosulfapyridine alone,probably via regulating immune status.
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Objective To observe the efficacy of omidazole in the treatment of trichomonal vaginitis.Methods 64 cases with trichomonal vaginitis were divided into two groups randomly.The experiment group(32 cases) was given ornidazole,while the control group was given metronidazole.Changes in symptoms laboratory tests,vaginal examination and adverse reactions were compared between the two groups.Results The efficacy of experiment group was significantly better than the control group( P ＜ 0.05).The experiment group's adverse reactions were sigenificantly less than the control group ( P ＜ 0.05 ).Conclusiou Ornidazole used in the treatment of trichormonal vaginitis shows a significant effect with long duration and less adverse reactions,so it's should be promoted.
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OBJECTIVE:To study the anti-tumor and anti-mutation effects of Shenlian granules. METHODS:The anti-mutation effect of Shenlian granules was tested by marrow cell micronucleus test and didymus chromosomal aberration test in mice, and the inhibitory effect of which on S180 and H22 grafting tumor was invesgated as well. RESULTS:Shenlian granules showed significant inhibitory effect on mutation of marrow cell micronucleus caused by cyclophosphamide and aberration of didymus chromosomal cell of mice caused by mitomycin as well as grafting tumor growth of S180 and H22. CONCLUSION:Shenlian granules can prevent DNA of both body cells and germ cells from damage but also inhibit grafting tumor growth in mice.
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OBJECTIVE:To determine the contents of vitamin B1 and vitamin B2 in duoweitie oral liquid by HPLC.METHODS:Samples were separated on C18,the mobile phase consisted of ion pair solution(0.24% of triethylamine solution containing 1.5 mmol?L-1 of sodium pentanesulfonate and 3.4 mmol?L-1 of sodium heptanesulfonate with pH adjusted to 3.0 by glacial acetic acid)-methanol(80∶20) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 254 nm and the sample size was 10 ?L.RESULTS:The linear ranges of vitamin B1 and vitamin B2 were 4.81～24.6(r=0.999 1) and 6.22～31.7 ?g?mL-1(r=0.999 8),respectively,and the average recovery rates were 103.3%(RSD=0.5%) and 99.5%(RSD=0.7%),respectively.CONCLUSION:This method is simple,accurate,sensitive and reliable,and suitable for the determination of vitamin B1 and vitamin B2 in duoweitie oral liquid.